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. 2019 Jul 4;9:9647. doi: 10.1038/s41598-019-46250-4

Figure 1.

Figure 1

Effect of D97S mutant expression on endogenous junctional protein. (A) Stable cell lines of mock or FLAG-tagged D97S mutant CLDN16 in MDCK cells were cultured for 72 h. The cell lysates were blotted with anti-FLAG, anti-CLDN1, anti-CLDN2, anti-CLDN4, anti-occludin (OCLN), anti-E-cadherin (E-cad), anti-ZO-1, anti-ZO-2, and anti-β-actin antibodies. The full-length blot images are shown in Supplementary Fig. S2. (B) The band densities of proteins are represented as the percentage of the values in mock cells. n = 4 in four independent experiments. **P < 0.01 compared with mock cells. NS P > 0.05.