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. 2019 Jul 4;9:9647. doi: 10.1038/s41598-019-46250-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of WT CLDN16 and the D97S mutant. (A) FLAG-tagged WT CLDN16 or the D97S mutant in MDCK cells were incubated with anti-FLAG (CLDN16) and anti-ZO-1 antibodies, followed by incubation with secondary antibodies. Merged images are shown on the right. (B) MDCK cells expressing the FLAG-tagged D97S mutant were incubated with anti-FLAG (CLDN16), anti-EEA1, anti-Rab7, anti-GM130, and anti-BiP antibodies or lysotracker. Enlarged images are shown on the right. The scale bar indicates 10 μm. (B) The colocalization of FLAG-tagged CLDN16 with organelle markers was shown as the percentage.