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. 2019 Jul 4;9:9647. doi: 10.1038/s41598-019-46250-4

Figure 7.

Figure 7

Effects of primaquine on paracellular permeability. FLAG-tagged WT CLDN16, D97S, or T303R mutant were transiently transfected in MDCK cells and cultured on transwell inserts for 96 h, then incubated for 2 h in the absence or presence of primaquine (PQ, 100 μM). (A,C) TER values were measured using a Millicell-ERS epithelial volt-ohmmeter and expressed as resistance (Ω·cm2). (B,D) The transepithelial permeability to Mg2+ for 1 h was measured using XB-1. n = 4 in four independent experiments. **P < 0.01, *P < 0.05, and NS P > 0.05 compared without PQ.