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. 2019 Jul 4;9:9710. doi: 10.1038/s41598-019-46130-x

Table 6.

Primers (DNA oligos) used for FBXO32, IL23A and TAGAP mRNA quantification by real-time RT qPCR, in Oryctolagus cuniculus species.

Genes Accession number Sequences of forward (FW) and reverse (RV) primers
FBXO32 XM_002710762 L-FBXO32-FW CCT-CCA-GCT-CTG-CAA-GCA-TT
L-FBXO32-RV AAA-GTC-CTG-GGG-TGA-AAG-TGA
IL23A XM_002711079 L-IL23A-FW TGG-GAC-CCA-TGG-AGC-TAC-TA
L-IL23A-RV TGC-AGG-CAG-AAC-TGA-GTG-TT
TAGAP XM_008263738 L-TAPAG-FW GGG-AAG-GTT-AAG-ACG-CTG-GT
L-TAPAG–RV GGT-GGA-CGT-GTC-TGA-ACT-GT
EIF4E2 ENSOCUG00000004534 L-EIF4E2-FW TGG-CAA-GTG-GAT-TAT-TCG-GC
L-EIF4E2-RV CAG-AGA-CCA-CAG-CCC-CAC-AG
RPL18 DQ403030 L-RPL18-FW CAA-CTC-CAC-GTT-CAA-CCA-GGT
L-RPL18-RV GGT-CTT-GTT-CTC-CCG-CCC

Endogenous transcripts, EIF4E2 and RPL18, were used as reference transcripts.