Table 1.
Relative abundance of dominant OTUs (after rarefaction) with phylogenetic affiliation of inflow and effluent samples based on the global SILVA alignment (SINA Aligner) for rRNA genes41.
| INFLOW | EFFLUENT | Genus | Affiliated species | Sequence Identity | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Feb | Apr | Jul | Oct | Feb | Apr | Jul | Oct | ||||
| OTU000121 | 0.92 | 0.78 | 2.26 | 1.37 | 0.04 | 0.09 | 0.13 | 0.27 | Acetoanaerobium ↓ | — | |
| OTU000039 | 4.23 | 2.53 | 3.07 | 5.46 | 0.51 | 0.13 | 1.03 | 1.19 | Acidovorax ↓ | — | |
| OTU000062 | 4.32 | 3.00 | 1.66 | 2.35 | 0.47 | 0.36 | 0.18 | 0.78 | Acidovorax ↓ | A. defluvii (Y18616.1) | 99.5% |
| OTU000055 | 6.58 | 3.27 | 2.60 | 2.53 | 0.25 | 0.98 | 0.67 | 0.63 | Acinetobacter ↓ | — | |
| OTU000065 | 2.39 | 4.43 | 1.30 | 1.63 | 0.34 | 0.40 | 0.63 | 0.76 | Aeromonas ↓ | A. media (X60410.1) | 97.8% |
| OTU000112 | 1.75 | 0.72 | 1.79 | 1.57 | 0.20 | 0.45 | 0.40 | 0.94 | Anaerosinus ↓ | A. glycerini (AJ010960.1) | 98.5% |
| OTU000414 | 0.00 | 0.00 | 0.00 | 0.00 | 0.78 | 1.25 | 1.59 | 1.30 | Aquabacterium ↑ | — | |
| OTU000015 | 2.55 | 8.28 | 3.20 | 8.42 | 0.90 | 1.14 | 0.90 | 3.69 | Arcobacter ↓ | A. cryaerophilus (FR682113.1) | 99.9% |
| OTU000307 | 0.45 | 0.07 | 0.34 | 0.51 | 0.18 | 0.40 | 0.11 | 1.10 | Bacteroides | B. graminisolvens (AB547643.1) | 99.8% |
| OTU000237 | 0.00 | 0.02 | 0.00 | 0.00 | 1.37 | 0.58 | 1.30 | 1.70 | Ca . Accumulibacter ↑ | — | |
| OTU000163 | 0.00 | 0.13 | 0.00 | 0.00 | 5.39 | 15.38 | 1.03 | 1.07 | Ca . Competibacter ↑ | — | |
| OTU000253 | 0.00 | 0.00 | 0.00 | 0.00 | 0.98 | 1.14 | 0.07 | 0.02 | Ca. Nitrotoga ↑ | Candidatus N. arctica (DQ839562.1) | 99.5% |
| OTU001077 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 1.63 | 0.02 | Chryseobacterium ↑ | — | |
| OTU000006 | 0.00 | 0.00 | 0.00 | 0.00 | 3.04 | 1.19 | 0.16 | 1.95 | Comamonadaceae uncl. ↑ | — | |
| OTU000040 | 0.00 | 0.07 | 0.00 | 0.00 | 6.20 | 2.26 | 0.11 | 0.78 | Comamonadaceae uncl. ↑ | — | |
| OTU000071 | 0.00 | 0.00 | 0.00 | 0.00 | 2.01 | 1.05 | 1.16 | 0.74 | Comamonadaceae uncl. ↑ | — | |
| OTU000188 | 0.65 | 0.87 | 1.01 | 0.96 | 0.00 | 0.02 | 0.02 | 0.09 | Comamonadaceae uncl. ↓ | — | |
| OTU000585 | 0.00 | 0.00 | 0.00 | 0.00 | 1.12 | 0.22 | 0.16 | 0.04 | Comamonadaceae uncl. ↑ | — | |
| OTU000031 | 4.90 | 2.75 | 6.11 | 5.55 | 0.43 | 0.27 | 0.16 | 1.54 | Comamonas ↓ | C. denitrificans (AF233880.1) | 99.9% |
| OTU000377 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 2.89 | 1.39 | Cupriavidus ↑ | — | |
| OTU000210 | 0.00 | 0.02 | 0.00 | 0.00 | 1.43 | 2.04 | 0.56 | 0.51 | Dechloromonas ↑ | — | |
| OTU000187 | 1.45 | 1.14 | 0.34 | 1.05 | 0.07 | 0.07 | 0.02 | 0.11 | Enterococcus ↓ | E. aquimarinus (EF204323.1) | 99.7% |
| OTU000153 | 1.14 | 1.07 | 0.25 | 1.54 | 0.00 | 0.07 | 0.00 | 0.04 | Faecalibacterium ↓ | F. prausnitzii (LQ500116.1) | 99.7% |
| OTU000381 | 0.00 | 0.00 | 0.00 | 0.00 | 0.69 | 0.72 | 1.63 | 0.74 | Geothrix ↑ | G. fermentans (U41563.1) | 97.8% |
| OTU000133 | 1.81 | 1.43 | 0.92 | 1.34 | 0.09 | 0.20 | 0.02 | 0.29 | Lachnospiraceae uncl. ↓ | — | |
| OTU000513 | 0.00 | 0.00 | 0.02 | 0.00 | 0.54 | 0.72 | 0.58 | 1.23 | Nitrosomonas ↑ | — | |
| OTU000519 | 0.00 | 0.00 | 0.00 | 0.00 | 0.22 | 0.13 | 1.19 | 1.05 | Nitrospira ↑ | — | |
| OTU000766 | 0.00 | 0.00 | 0.00 | 0.00 | 0.09 | 0.18 | 1.28 | 0.34 | OM27 clade ↑ | — | |
| OTU000467 | 0.34 | 0.60 | 1.75 | 0.09 | 0.02 | 0.00 | 0.02 | 0.00 | Paracoccus ↓ | P. lutimaris (KJ451483.1) | 99.2% |
| OTU000209 | 0.22 | 0.45 | 0.25 | 0.81 | 1.05 | 0.40 | 0.60 | 1.19 | Peptostreptococcaceae uncl. ↑ | — | |
| OTU000109 | 2.55 | 1.81 | 2.53 | 0.83 | 0.25 | 0.47 | 0.13 | 0.56 | Proteocatella ↓ | — | |
| OTU000466 | 0.00 | 0.00 | 0.00 | 0.00 | 4.23 | 0.72 | 0.02 | 0.16 | Rhodoferax ↑ | — | |
| OTU000339 | 0.00 | 0.02 | 0.00 | 0.00 | 0.45 | 0.27 | 1.23 | 1.59 | Run-SP154 uncl. ↑ | — | |
| OTU000756 | 0.00 | 0.00 | 0.00 | 0.00 | 2.13 | 0.78 | 0.47 | 0.13 | Simplicispira ↑ | S. limi (LC177120.1) | 98.1% |
| OTU000123 | 0.51 | 0.96 | 5.35 | 0.78 | 0.07 | 0.04 | 0.09 | 0.07 | Streptococcus ↓ | S. parasuis (AB936273.1) | 97.7% |
| OTU000387 | 0.11 | 0.11 | 2.22 | 0.27 | 0.04 | 0.04 | 0.02 | 0.04 | Streptococcus ↓ | — | |
| OTU000685 | 0.04 | 0.04 | 1.48 | 0.11 | 0.00 | 0.00 | 0.00 | 0.00 | Streptococcus ↓ | — | |
| OTU000873 | 0.02 | 0.00 | 1.12 | 0.00 | 0.02 | 0.00 | 0.02 | 0.02 | Streptococcus ↓ | — | |
| OTU000128 | 1.84 | 1.50 | 0.74 | 1.10 | 0.38 | 0.47 | 0.11 | 0.47 | Subdoligranulum ↓ | — | |
| OTU000165 | 1.16 | 1.05 | 0.45 | 0.96 | 0.22 | 0.18 | 0.09 | 0.47 | Subdoligranulum ↓ | — | |
| OTU000078 | 0.27 | 1.28 | 1.84 | 1.03 | 0.92 | 1.16 | 4.07 | 1.88 | Thauera ↑ | — | |
| OTU000017 | 9.65 | 6.51 | 2.17 | 6.78 | 1.10 | 1.01 | 0.11 | 1.28 | Trichococcus ↓ | T. flocculiformis (JF505981.1) | 99.7% |
| OTU000098 | 0.00 | 0.04 | 0.00 | 0.00 | 0.78 | 0.85 | 1.19 | 0.67 | Saprospiraceae uncl. ↑ | — | |
| OTU001461 | 0.00 | 0.00 | 0.00 | 0.00 | 0.40 | 1.68 | 0.02 | 0.00 | Neisseriaceae uncl. ↑ | — | |
| OTU000099 | 2.57 | 2.71 | 3.02 | 0.94 | 0.13 | 0.07 | 0.02 | 0.07 | Uruburuella ↓ | U. suis (AJ586614.1) | 99.9% |
| OTU000397 | 0.38 | 0.29 | 1.37 | 0.11 | 0.02 | 0.02 | 0.02 | 0.11 | Veillonella ↓ | — | |
| OTU000829 | 0.00 | 0.04 | 0.00 | 0.00 | 0.76 | 1.05 | 0.78 | 0.18 | Zoogloea ↑ | — | |
| Sum | 52.8 | 48.0 | 49.2 | 48.1 | 40.3 | 40.7 | 28.6 | 33.2 | |||
Only OTUs contributing at least in one sample more than 1.0% are shown for the samples collected in February (Feb), April (Apr), July (Jul) and October (Oct). OTUs that significantly change in abundance between inflow and effluent are indicated in bold (all are highly significant with p-values < 0.001). Arrows indicate an increase (↑) or decrease (↓) from inflow to effluent.