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. 2019 Jul 4;9:9715. doi: 10.1038/s41598-019-46064-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of HSPB8 inhibits migration of metastatic prostate cancer cells. (a) Expression of HOXB13, AR and HSPB8 in various cell line models. RWPE-1 is AR-low; VCaP, LNCaP, C4-2B and 22Rv1 are AR-positive; PC3 and DU145 are AR-negative cell lines. (b) C4-2B cells were transfected with 4 µg of GFP-Vector or GFP-HSPB8. Whole cell lysates were immunoblotted with anti-HSPB8 antibody. Actin is a normalization control. (c) Stratification of PC cell lines based on their HOXB13, AR and AR-V7 expression. (d,e) C4-2B, PC3 and 22Rv1 cells were transfected with 4 µg of GFP-Vector or GFP-HSPB8. Transwell cell migration assay was performed using fluroblock inserts. (d) After 16 h, cells were visualized and captured using the EVOS-M5000 microscope. Scale bar 300 µm. (e) Quantitative analysis of GFP-positive migrated cells. n = 2 independent replicates. ± SEM. **p < 0.01.