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. 2019 Jul 4;9:9711. doi: 10.1038/s41598-019-45910-9

Figure 2.

Figure 2

Validation of EMC as a proviral factor for DENV2 and establishment of EMC4-modified cell lines. (A) Cells were transfected with sgRNAs to knockout the indicated genes in HuH-7 cells. Subsequently cells were infected with DENV2 and rates of infection measured by high content imaging. sgRNAs targeting GFP served as negative controls. (B) Western blot characterization of clonal, gene-edited HuH-7 lines. Cells containing an HA-tag knocked into EMC4 alleles were initially generated and a single clone was used to generate two clonal HA-EMC4 knockout lines. Levels of other EMC subunits are shown for each cell line. WT indicates parental HuH-7 cells. (*p < 0.05). The western blots are from the same gel and the image was cropped to show EMC4 and HA-EMC4, and ß-actin (loading control).