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. 2019 Jul 4;9:9711. doi: 10.1038/s41598-019-45910-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EMC4 is required for ZIKV replicon RNA replication and protein expression. (A) Structure of the ZIKV replicon is shown. (B) Measurements of replicon RNA levels in cells transfected with non-targeting (NT) control and EMC4 siRNAs. (C) Western blot analysis of the indicated proteins is shown from triplicate samples. (D) Quantitative analysis of proteins levels from panel (C). (E) Cells were treated with CHX for the indicated time points and protein samples harvested. Western blot analysis of the indicated proteins is shown. (F) Quantitative analysis of protein levels from two independent experiments performed in triplicate is shown. (*p < 0.05; **p < 0.005). In panels (C,E) western blots are each from one gel and the images were cropped to show mature EMC4, RLuc, and mature viral proteins (NS2B, NS3 and NS4B), and ß-actin or tubulin respectively (as loading controls).