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. 2019 Jul 4;10:2968. doi: 10.1038/s41467-019-10830-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Neuronal p38α and p38β MAPK are not necessary for NMDAR-dependent LTD. a Immunohistochemistry of hippocampal sections from WT, p38β^−/−, and p38β^−/−/p38α (neuronal)^−/− animals with anti-MAP2 antibody (green) and anti-p38α antibody (red). SP: stratum pyramidale (indicated with an arrowhead in middle panels). SR: stratum radiatum. SLM: stratum lacunosum moleculare. Scale bars, 200 μm. b Western blot analysis of p38α and p38β protein levels from WT, p38β^−/−, and p38β^−/−/p38α (neuronal)^−/− hippocampus. Actin served as a loading control. c Relative amount in percentage of p38β and p38α from WT (black bars, n = 6), p38β^−/−(green bars, n = 4), and p38β^−/−/ p38α (neuronal)^−/− (red bars, n = 4) from western blots as the one shown in b. Kruskal–Wallis test followed by Dunn’s test (***P < 0.001). d Input–output curves presenting fEPSP slope from acute hippocampal slices in response to increasing stimulus strength (n > 39 slices from n > 7 mice). No differences were found by two-way ANOVA (F(2, 240); P = 0.73). e (Left) Time course of relative changes in fEPSP slope before and after LFS in acute hippocampal slices from WT (black, n = 11 from 7 mice), p38β^−/−/p38α neuronal)^−/− (red, n = 9 slices from 7 mice) and p38β^−/− (green, n = 11 from 7 mice) knockout mice. (Right) Summary of results from the end of the time courses shown on the left. Differences between groups were determined by Kruskal–Wallis test followed by Dunn’s test (*P < 0.05). Wilcoxon statistical test was used to analyze LTD expression with respect to baseline (^##P < 0.01)