Table 4.
Primers used in quantitative PCR (qPCR).
| Forward primer Sequence (5′ > 3′) | Reverse primer Sequence (5′ > 3′) | Amplicon Size (bp) | R2 | Efficiency | |
|---|---|---|---|---|---|
| GAPDH | GCAAATTCCATGGCACCGT | TCGCCCCACTTGATTTTGG | 106 | 0.999 | 94.3~100.8% |
| PLA2G2a | CAACGGATCGCTGCTGTGTC | GCCACATCCACGTTTCTCCA | 65 | 0.998 | 98.6% |
| PLA 2 G4a | ACAAAGCAGAGGCTCCACAA | TTCAGTGCCTTTGGGTTCGT | 115 | 0.992 | 98.0% |
| PLA 2 G5 | TCCTGGCTTGTAGTGTGCCT | CCGTAGTTTGTCAGGGCGTT | 97 | 0.994 | 97.5% |
| IL-1β | TGCTCTGGGATTCTCTTCAGC | ATTGCCACTGTAATAAGCCATCA | 107 | 0.996 | 98.3% |
| IL-6 | TGAGGAGACTTGCCTGGTGA | CACAGCTCTGGCTTGTTCCT | 109 | 0.997 | 96.3% |
| TNF-α | ATGTTGTAGCAAACCCTCAAGC | TCTCTCAGCTCCACGCCATT | 91 | 0.99 | 94.3% |
GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; PLA2G2a: Phospholipase A2 group 2a; PLA2G4a: Phospholipase A2 group 4a; PLA2G5: Phospholipase A2 group 5; IL-1β: Interleukin 1 beta; IL-6: Interleukin 6; TNF-α: Tumor necrosis factor alpha.