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. 2019 Jul 4;9:9720. doi: 10.1038/s41598-019-45521-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Normal and amputated mouse digit tips showing the completeness of tissue regeneration after amputation. We performed the amputations at 3 days after birth (dab). Non-amputated digit sections at 3 (a), 17 (b), 33 (c) and 123 (d) dab were used as control for comparison. Digit tips after distal amputation at 0 (a’), 15 (b’), 30 (c’) and 120 (d’) dpa. Digit tip after oblique distal amputation at 0 (a”), 15 (b”), 30 (c”) and 120 (d”) dpa. Mallory trichrome stain (a – c”) and bright field images (d – d”). Arrows in c’ and c” indicate the presence and absence of the fat pad, respectively (Scale bars, 100 μm).