Table 5.

Primer sequences of flavonoid and chlorogenic acid biosynthetic pathways genes.

Primer	Solyc ID	Abver	Sequence (5′-3′)
Phenylalanine ammonia-lyase 1	Solyc10g086180.1	PALF PALR	ACGGGTTGCCATCTAATCTGACA CGAGCAATAAGAAGCCATCGCAAT
Cinnamic acid 4-hydroxylase	Solyc05g047530.2	C4HF C4HR	CCC AGT TTT TGG AAA TTG GCT TCA GCC CCA TTC TAA GCA AGA GAA CAT C
Chalcone synthase	Solyc05g053550.2	CHSF CHSR	CAC CGT GGA GGA GTA TCG TAA GGC TGA TCA ACA CAG TTG GAA GGC G
Chalcone isomerase 2	Solyc02g062170	CHIF CHIR	GGC AGG CCA TTG AAA AGT TCC CTA ATC GTC AAT GAT CCA AGC GG
Flavanone 3-hydroxylase	Solyc02g083860.2	F3HF F3HR	CCA AGG CAT GTG TGG ATA TGG ACC CCT GGA TCA GTA TGT CGT TCA GCC
Flavonol synthase 1	Solyc11g013110.1	FLSF FLSR	CCT CCT TCC TAC AGG GAA GCA AA CAA GCC CAA GTG ACA AGC TCC TAA
Dihydroflavonol 4-reductase	Solyc02g085020	DFRF DFRR	TCA CAG GAG CAG CTG GAT TTA TCG TCA GGA TCA CGA ACA GTA GCA TGG
Flavonoid 3′ hydroxylase	Solyc11g066580	F3′HF F3′HR	TGG GTA TAC CCA AAC TCA TTC CG AAA AGC CCA AAG TTG ATG TGA AAG G
Anthocyanin 1 transcription factor	Solyc10g086260	AN1F AN1R	CCT CAA CCT CAG AAA TTC AGA AGC TCG TTG TTGTTG TCG TTC GAT GC
Anthocyanin 2 transcription factor	Solyc10g086250	AN2F AN2R	ACAAGATGCCACTTTCCTTCACC TGTGCATCGTTGGGAGTTAGG
Hydroxycinnamoyl Co A shikimate hydroxycinnamoyl transferase	Solyc06g074710	HCTF HCTR	TCT CCA ACC CCT TTT AAC GAA CC CAA CTT GTC CTT CTA CCA CAG GGA A
Hydroxycinnamoyl Co A quinate hydroxycinnamoyl transferase	Solycg07g005760	HQTF HQTR	CCC AAT GGC TGG AAG ATT AGC TA CAT GAA TCA CTT TCA GCC TCA ACA A
p-coumarate 3-hydroxylase	Solyc10g078240	C3HF C3HR	TTG GTG GCT ACG ACA TTC CTA AGG GGT CTG AAC TCC AAT GGG TTA TTC C