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. 2019 Jul 4;9:9713. doi: 10.1038/s41598-019-46085-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cryo SEM images of mucus. (A) Image of a drop showing a pore mesh. Scale bar 10 μm. (B) Histogram of pore sizes in mucus, as extracted from panel (A). (C) Mucus thread deposited vertically. Scale bar 100 μm. There is a vertical alignment of the mucus micro-structure, parallel to the orientation of the thread. The borders, however, show wrinkles almost perpendicular to the main direction. (D) Wrinkles at the edge of the thread, oriented perpendicular to the walls. Scale bar 10 μm. (E) Blowup of (C) showing the main vertical alignment close to the center of the thread. Scale bar 10 μm. Some ice crystals are present in panels (C) and (D).