Figure 3.

Collagen induces tumor progression through integrin α2β1. A, Relative gene expression of integrin α2, α1, α10, and α10 in collagen‐treated MB49 cells (0.1 μg/mL) for 72 h or not (PBS). B, Western blot analysis of integrin α2β1 and actin in MB49 cells treated with collagen (0.1 μg/mL) for 72 h. C, Relative colony numbers and cell numbers of MB49 cells treated with collagen (0.1 μg/mL) or collagen (0.1 μg/mL) combined with E7820 (0.05 μg/mL) for 72 h. D, Relative colony numbers and cell numbers of T24 cells treated with collagen (0.1 μg/mL) or collagen (0.1 μg/mL) combined with E7820 (0.05 μg/mL) for 72 h. E, Bladder tumor weights of mice treated with collagen (1 μg) or collagen (1 μg) combined with E7820 (50 μg) on days 4 and 8. Error bars, mean ± SEM. *P < .05; **P < .01. n.s, not statistically significant