Figure 3.

Elimination of CRK reduces expression of ErbB2 and ErbB3 in exosomes derived from bladder cancer cells. A, An equivalent amount of protein (1 μg) from exosomes derived from UM‐UC‐3 cells (empty [Emp‐1] and CRKi) were subjected to SDS‐PAGE, followed by silver staining. B, In exosomes derived from UM‐UC‐3 cells (parent, empty, and CRKi), expression levels of the indicated proteins were examined by immunoblotting. CD63 is an exosomal marker and was used as a loading control. EGFR, epidermal growth factor receptor; FAK, focal adhesion kinase