Quantification of PAR chains according to irradiation dose, time point, and PARPi.
A. CH2879 cells were irradiated with X-rays (0, 0.5 Gy, 1 Gy, and 2 Gy), and PAR chains were analyzed by western blot following irradiation at various time points (0, 1, 3, 6, 8, 24, and 48 h); actin served as the loading control. B. CH2879 cells were irradiated with X-rays (0, 0.5 Gy, 1 Gy, and 2 Gy), and PAR chains were analyzed by western blot following irradiation with or without PARPi at 24 h after irradiation; actine served as the loading control.