Skip to main content
. 2019 Jun 18;110(7):2296–2308. doi: 10.1111/cas.14041

Figure 1.

Figure 1

Vasohibin‐2 (Vash2) was upregulated during pancreatic ductal adenocarcinoma (PDAC) progression and promoted PDAC cell migration. A, Representative images of H&E staining and comparison of Vash2 mRNA expression using qRT‐PCR analysis in normal pancreatic tissue (n = 5), PanIN (n = 5) and PDAC (n = 7) from KPC mice. B‐D, Comparison of (B) Vash2 mRNA expression (n = 3), (C) proliferation using WST‐1 assay (n = 2) and (D) migration using the Boyden chamber (n = 3) in KPC stable clones expressing control vector (KPC/shControl) or mouse Vash2 shRNA vector (KPC/shVash2#1, KPC/shVash2#2). E, F, Comparison of (E) VASH2 mRNA expression, (F) migration in PANC‐1 cells stably transfected with control vector (PANC‐1/shControl) or human Vash2 shRNA vector (PANC‐1/shVASH2#1, PANC‐1/shVASH2#2) (n = 3). G, H, Comparison of (G) VASH2 mRNA expression and (H) migration of SUIT‐2 cells infected with LacZ adenovirus (AdLacZ) or human VASH2 adenovirus (AdVASH2) (n = 3). Error bars represent SD. Scale bars are 500 μm (A) and 100 μm (D, F, H). *P < .05, **P < .01