Figure EV4. CD4 T_HD cells in NSCLC patients are mainly non‐senescent memory subsets.

• A
  Scatter plot graphs of the percentage of memory phenotypes in baseline CD4 T_HD cells according to CD62L‐CD45RA expression (% CD45RA^negative CD62L^positive central‐memory + % CD45RA^negative CD62L^negative effector‐memory cells) in a sample of healthy donors (n = 18), G1 (n = 12) and G2 (n = 19) patients. Relevant statistical comparisons are shown by one‐way ANOVA followed by Tukey's test.
• B, C
  Expression of the genotoxic damage makers H2AX (B) and CD57 (C) by flow cytometry in CD4 T‐cell subsets from an aged‐matched healthy donor, and NSCLC G1 and G2 patients as indicated. Percentage of positivity and mean fluorescent intensities are indicated for each population. Top, histogram analysis within CD27⁺ CD28⁺ CD4 T cells, and bottom, CD27^negative CD28^low/negative counterparts as indicated. US, unstained control.