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. 2019 Jun 6;11(7):e10201. doi: 10.15252/emmm.201810201

Figure 5. Down‐regulation of DKC1 mRNA in PARN KO cells is dependent on p53.

Figure 5

  1. HT1080 (WT) and HT1080PARN KO (KO) cells stably transfected with GFP plasmid (empty) were transfected with siRNAs against p53 for 72h before RNA extraction and qRT–PCR. siRNAs against luciferase (siLuci) were used as control. p53 expression levels were normalized first to ACTB and then to WT/empty/siLuci cells. Experiment was performed in triplicate. Error bars indicate s.e.m.
  2. Cells were treated as in (A), and transcript levels of the indicated genes were measured by qRT–PCR. cDNA expression levels were normalized first to ACTB and then to WT/empty/siLuci cells. Experiment was performed in triplicate. Error bars indicate s.e.m.
  3. qRT–PCR analyses of the indicated genes were performed on RNA extracted from P1's primary or SV40T‐transformed fibroblasts. cDNA expression levels were normalized to primary fibroblasts. Two independent RNA extractions were performed. Error bars indicate s.e.m.