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. 2019 Jun 6;11(7):e10201. doi: 10.15252/emmm.201810201

Figure 7. The early embryonic lethality of Parn KO is not rescued in p53 −/− mice.

Figure 7

  • A
    Western blot analysis of Parn expression in control and Parn +/− MEFs.
  • B
    Northern blot analysis of total RNAs from WT and Parn +/− MEFs.
  • C
    Schematic representation of mouse rRNA precursors and position of DNA probes used in Northern blot.
  • D
    Frequencies of expected and observed newborns of the indicated genotype obtained from Parn +/− intercrosses. n indicates the number of animals analyzed. Statistical significance (P = 1.5417E‐08) of the differences between the observed and expected genotype distributions was assessed by chi‐square test.
  • E
    Frequencies of expected and observed E11.5 embryos of the indicated genotype obtained from Parn +/− intercrosses. n indicates the number of animals analyzed. Statistical significance (P = 0.02535) of the differences between the observed and expected genotype distributions was assessed by chi‐square test.
  • F, G
    p53 knock‐out does not rescue Parn −/−. No Parn −/− p53 −/− mouse was born from various combinations of Parn +/− and either p53 +/− or p53 −/− crosses (49 and 56 pups, respectively). Statistical significance (P = 0.000886) of the differences between the observed and expected genotype distributions was assessed by chi‐square test.