Figure EV1. Validation of the novel pathways of TRF1 regulation.

• A
  Representative image of the pathways included in Reactome database.
• B
  Quantification of TRF1 nuclear fluorescence in CHA9‐3 lung cancer cells treated with DMSO or structurally different MEK inhibitors for 24 h at 1 μM. Data are representative of n = 2 biological replicates
• C
  Quantification of TRF1 nuclear fluorescence in CHA9‐3 lung cancer cells treated with DMSO or structurally different ERK inhibitors for 24 h at 1 μM. Data are representative of n = 2 biological replicates
• D
  Western blot images (up) and p‐ERK protein levels (down) of CHA9‐3 lung cancer cells treated with the indicated compounds for 24 h at 1 μM. Data are representative of n = 2 biological replicates
• E
  Quantification of TRF1 nuclear fluorescence in CHA9‐3 lung cancer cells treated with DMSO or structurally different HSP90 inhibitors for 24 h at 1 μM. Data are representative of n = 2 biological replicates
• F
  Quantification of TRF1 nuclear fluorescence in CHA9‐3 lung cancer cells treated with DMSO or different tubulin agents for 24 h at 1 μM. Data are representative of n = 2 biological replicates.

Data information: Data are represented as mean ± SEM. Significant differences using unpaired t‐test are indicated by *P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure.