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Y Chromosome Fluorescent in Situ Hybridization of the Explanted Grafts
The male seeded cells (Y chromosome [Y chr] positive) were detected over the scaffold before implantation and after 24 h, 1 week, and 2 weeks. The number of seeded cells decreased with time in vivo. No seeded cells were observed at 4.5 months. Scale bars, 50 μm. Dapi = 4′,6-diamidino-2-phenylindole.
