Table 1.
Antibodies for Immunohistochemistry
| Primary Antibody | Primary Antibody Dilution | Manufacturer | Secondary Antibody | Secondary Antibody Dilution | Manufacturer |
|---|---|---|---|---|---|
| Mouse anti–alpha–sarcomeric actinin | 1:100 | Abcam | Goat antimouse–Alexa Fluor 488 | 1:400 | Abcam |
| Mouse anti–alpha–smooth muscle actin | 1:200 | Abcam | Goat antimouse–Alexa Fluor 488 | 1:400 | Abcam |
| Mouse anti–cardiac myosin heavy chain | 1:150 | Thermo Fisher Scientific | Goat antimouse–Alexa Fluor 488 | 1:400 | Abcam |
| Rabbit anti–connexin-43 | 1:100 | Santa Cruz Biotechnology | Goat antirabbit–Alexa Fluor 546 | 1:400 | Abcam |
| Mouse anti–discoidin domain receptor 2 | 1:100 | Santa Cruz Biotechnology | Goat antimouse–Alexa Fluor 488 | 1:400 | Abcam |
| Isolectin B4-biotin | 1:100 | Life Technologies | Streptavidin–Alexa Fluor 488 | 1:200 | Life Technologies |
| Rabbit anti-Ki67 | 1:200 | Abcam | Goat antirabbit–Alexa Fluor 546 | 1:400 | Abcam |
| Mouse anti–metalloproteinase 1 | 1:200 | Abcam | Goat antimouse–Alexa Fluor 488 | 1:400 | Abcam |
| Mouse anti–smooth muscle myosin heavy chain | 1:100 | Dako | Goat antimouse-Cy3 | 1:300 | Jackson ImmunoResearch Laboratories |
Primary antibodies used for immunofluorescent staining and respective secondary antibodies used for detection.