Figure 4. TFL₄₅₇ preserves additional survival proteins downstream TrkB signaling in excitotoxicity.

• A
  Dose‐dependent TFL₄₅₇ effects. Cultures incubated with TMyc o TFL₄₅₇ (5, 15, or 25 μM, 30 min) were treated with NMDA (2 h) and compared to cells without peptide or NMDA.
• B
  Time‐course TFL₄₅₇ effects. Cells preincubated with peptides (25 μM, 30 min) were treated with NMDA (0–6 h).
• C
  Quantitation of peptide effects. Normalized protein levels are presented relative to values obtained in cells incubated with the same peptide but no NMDA. Means ± SEM are given. Results were analyzed by a two‐way ANOVA test followed by post hoc Bonferroni test, comparing TMyc‐ or TFL₄₅₇‐treated cells for each time point (TrkB‐FL, ***P = 0.0009 and *P = 0.02; CREB, *P = 0.0149 and *P = 0.0148; pCREB, ***P = 0.0007 and **P = 0.0012; MEF2D, **P = 0.0023 and **P = 0.0047. All values are, respectively, for 2 or 4 h; n = 6).

Source data are available online for this figure.