Figure 7. TFL₄₅₇ counteracts TrkB‐FL downregulation in ischemia and reduces infarct volume and neurological damage.

• A
  Confirmation of Bio‐TFL₄₅₇ delivery to mice cortex. Biotinylated TFL₄₅₇ (Bio‐TFL₄₅₇, 4 nmol/g, retro‐orbitally injected) was detected in coronal sections stained with NeuN and DAPI. Representative confocal microscopy images of cortical areas correspond to single sections. Scale bar, 10 μm.
• B
  Timeline to analyze in vivo effects of MTMyc and MTFL₄₅₇. Mice subjected to ischemia as before were retro‐orbitally injected with CPPs (10 nmol/g) 10 min after damage initiation.
• C
  Representative analysis of TrkB‐FL at 3 h of damage. Comparison of the infarcted area (I) and the corresponding contralateral region (C) is shown.
• D
  Quantitation of TrkB‐FL levels_. Means ± SEM of normalized TrkB‐FL levels in the infarcted area relative to the corresponding contralateral region are presented and results analyzed by Student's t‐test (*P = 0.021; n = 3).
• E
  Representative 1 mm brain coronal slices stained with TTC after 24 h of insult corresponding to animals injected with MTMyc or MTFL₄₅₇.
• F
  Infarct volume of animals injected with MTMyc or MTFL₄₅₇ expressed as a percentage of the hemisphere volume. Means ± SEM are given. Differences were analyzed by Student's t‐test (***P = 0.00008; n = 14). Results for MTFL₄₅₇ are also expressed as percentage of the infarct volume in animals injected with MTMyc.
• G
  Evaluation of balance and motor coordination. Number of contralateral hind paw slips were measured after 24 h of damage. Data spread is presented by box and whisker plots showing interquartile range, median, minimum, and maximum values. Means (dots) ± SEM were also calculated and analyzed by Student's t‐test (*P = 0.04; n = 11).

Source data are available online for this figure.