Figure 2.

In subcutaneous adipose tissue, ECHDC3 is primarily expressed in adipocytes and ECHDC3 expression is induced with adipocyte differentiation. Results from the qRT-PCR analysis of expression of ECHDC3 mRNA (normalized to RPLP0 [36B4] endogenous control gene) are shown. A: Relative expression of ECHDC3 in pooled RNA samples from stromal vascular fractions (SVFs) compared with the adipocyte fraction (AF) of subcutaneous adipose tissue in African Americans (data for two technical replicates of the pooled RNA samples from 5–6 individual donors are shown). Expression of ECHDC3 at different stages of in vitro differentiation of hADSCs (B) and SGBS preadipocytes (C). Data are shown for independent biological triplicates at each stage of differentiation. d and D, days; H, hours.