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. 2019 Apr 22;68(7):1508–1522. doi: 10.2337/db18-1229

Figure 3.

Figure 3

Knocking down of ECHDC3 gene in SGBS adipocytes affects insulin sensitivity. A: Lentiviral particle-mediated delivery of gene-specific shRNA expression vector stably knocks down ECHDC3 mRNA levels in SGBS adipocytes. Results from the qRT-PCR analysis of expression of ECHDC3 mRNA (normalized to RPLP0 [36B4] endogenous control gene) in SGBS cells stably expressing control shRNA or ECHDC3 shRNA at 14 days post–initiation of differentiation are shown. Bar graph indicates the mean ± SD of six biological replicates for each condition from two independent experiments. B: Basal and insulin-stimulated glucose uptake in control shRNA and ECHDC3 shRNA (ECHDC3-KD) SGBS cells at 14 days of differentiation. Bar graph indicates the mean ± SD of biological triplicates for each condition. Western blot analysis at day 14 of differentiated control shRNA and ECHDC3-KD SGBS cells using antibodies specific for pAKt, total Akt, and GAPDH at basal and insulin-stimulated (100 nmol/L for 15min) conditions (C) and quantified data (mean ± SD of biological triplicates) from scanning of a representative (selected from two independent experiments) Western blot (D) are shown. DPM, disintegrations per minute.