Table II.

Mutations of bile cell-free DNA from ten biliary tract cancer patients.

Patient ID	SNV/Indel			CNV
Patient #1	TP53	p.Q38Pfs*5	0.498a	CDK12	Gain
	ERBB2	p.D769Y	0.004	ERBB2	Gain
	PIK3CA	c.1665-1G>T	0.004	CCNE1	Gain
				CDKN2A	Loss
				ZNF217	Gain
Patient #2	TP53	p.C275F	0.037	FGFR2	Gain
	TP53	p.P27Lfs*17	0.012
	ARID2	p.Q794Cfs*45	0.014
	KRAS	p.A146V	0.006
	TP53	p.E180K	0.022
	SETD2	p.M118I	0.015
Patient #3	TP53	p.P151T	0.022	FLT1	Gain
	NOTCH2	p.T235S	0.011
Patient #4	TP53	p.V157F	0.062	CCNE1	Gain
	FLT4	p.A622T	0.010	CDKN2A	Loss
	NOTCH1	p.R1824Q	0.492
	KMT2A	p.G3131S	0.467
	NOTCH2	p.R29Q	0.028
Patient #5	TP53	c.783-2_821del	0.050
	CDKN2A	p.V82E	0.169
	KRAS	p.I36M	0.246
	IRS2	p.Q1206R	0.592
	EP300	p.P881S	0.423
	FAT1	p.D850E	0.129
Patient #6	TP53	p.M237I	0.760	CDK6	Gain
	PDGFRA	p.L216I	0.516	MET	Gain
	NF1	p.E649*	0.710	BRAF	Gain
	RNF43	p.L706Cfs*20	0.471	PIK3CA	Gain
	FGFR3	p.K413R	0.320	ALK	Gain
	FGFR1	p.G35R	0.109
	SPTA1	p.N2076K	0.107
	PIK3R1	p.N564D	0.034
	AR	p.K778M	0.014
Patient #7	KRAS	p.G12D	0.016
	ATM	p.A2626V	0.012
	NOTCH1	p.R592H	0.486
	KMT2A	p.P658P	0.483
	VEGFA	p.T8Rfs*78	0.385
Patient #8	TP53	p.R248W	0.533	FGFR3	Gain
	TSC1	p.R190H	0.130	VEGFA	Gain
	ATR	p.M211I	0.441	MYC	Gain
	BRCA1	p.R762S	0.197	ERBB3	Gain
				CDKN1B	Gain
				APC	Gain
				ROS1	Gain
Patient #9				MRE11A	Gain
				MTOR	Loss
				CDKN2A	Loss
				STK11	Loss
				ERBB2	Gain
				CCNE1	Gain
				CDK6	Gain
Patient #10				CDKN1B	Gain
				CCNE1	Gain
				ZNF217	Gain

^aMutation allele frequency in bile cell-free DNA sample. SNV/Indel, single nucleotide variation/insertion and deletion; CNV, copy number variation.