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. 2019 Jun 6;42(2):561–570. doi: 10.3892/or.2019.7187

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Copyright: © Geng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — DNA-PKcs inhibition increases the sensitivity of BGC823 and MGC803 cells to IR. (A) BGC823 and MGC803 cells were exposed to increasing concentrations of NU7441 (a DNA-PKcs inhibitor) and the IC₅₀ was calculated. (B) BGC823 and (C) MGC803 cells treated with or without NU7441 (0.1 µM) were exposed to increasing doses of IR (0, 2, 4, 6 and 8 Gy). (D) The protein expression levels of cleaved-caspase3 and γH2AX were elevated upon NU7441 (0.1 µM) treatment in BGC823 and MGC803 cells following IR (4 Gy). (E) Quantification of protein expression levels. **P<0.01 and ***P<0.0001 vs. DMSO. (F) NU7441 (0.1 µM) significantly elevated IR induced cell apoptosis of BGC823 and MGC803 cells. (G) Quantification of cell apoptotic rates. **P<0.01 and ***P<0.0001. DNA-PKcs, DNA-dependent protein kinase catalytic subunit; DMSO, dimethyl sulfoxide; IR, ionizing radiation; γH2AX, γ H2A histone family member X; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Figure 4. — DNA-PKcs inhibition increases the sensitivity of BGC823 and MGC803 cells to IR. (A) BGC823 and MGC803 cells were exposed to increasing concentrations of NU7441 (a DNA-PKcs inhibitor) and the IC₅₀ was calculated. (B) BGC823 and (C) MGC803 cells treated with or without NU7441 (0.1 µM) were exposed to increasing doses of IR (0, 2, 4, 6 and 8 Gy). (D) The protein expression levels of cleaved-caspase3 and γH2AX were elevated upon NU7441 (0.1 µM) treatment in BGC823 and MGC803 cells following IR (4 Gy). (E) Quantification of protein expression levels. **P<0.01 and ***P<0.0001 vs. DMSO. (F) NU7441 (0.1 µM) significantly elevated IR induced cell apoptosis of BGC823 and MGC803 cells. (G) Quantification of cell apoptotic rates. **P<0.01 and ***P<0.0001. DNA-PKcs, DNA-dependent protein kinase catalytic subunit; DMSO, dimethyl sulfoxide; IR, ionizing radiation; γH2AX, γ H2A histone family member X; PI, propidium iodide; FITC, fluorescein isothiocyanate.