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. 2019 Jun 6;42(2):561–570. doi: 10.3892/or.2019.7187

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Copyright: © Geng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Combination of DNA-dependent protein kinase catalytic subunit inhibition and CK2 inhibition further increases sensitivity of gastric cancer cells to IR. (A) BGC823 and (B) MGC803 cells treated with NU7441 (0.1 µM), or a combination of NU7441 (0.1 µM) and CX4945 (0.5 µM; a CK2 inhibitor), were exposed to increasing doses of IR (0, 2, 4, 6 and 8 Gy). (C) Western blotting showed that the combination of NU7441 (0.1 µM) and CX4945 (0.5 µM) induced a significant elevation of cleaved-caspase3 and γH2AX protein expression levels compared with treatment with NU7441 (0.1 µM) alone in BGC823 and MGC803 cells. (D) Quantification of protein expression levels. **P<0.01 and ***P<0.0001 vs. NU7441. (E) CX4945 further significantly elevated NU7441 induced cell apoptosis of GC823 cells and MGC803 cells. (F) Quantification of cell apoptosis rates. **P<0.01 and ***P<0.0001. CK2, casein kinase 2; IR, ionizing radiation; γH2AX, γ H2A histone family member X; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Figure 6. — Combination of DNA-dependent protein kinase catalytic subunit inhibition and CK2 inhibition further increases sensitivity of gastric cancer cells to IR. (A) BGC823 and (B) MGC803 cells treated with NU7441 (0.1 µM), or a combination of NU7441 (0.1 µM) and CX4945 (0.5 µM; a CK2 inhibitor), were exposed to increasing doses of IR (0, 2, 4, 6 and 8 Gy). (C) Western blotting showed that the combination of NU7441 (0.1 µM) and CX4945 (0.5 µM) induced a significant elevation of cleaved-caspase3 and γH2AX protein expression levels compared with treatment with NU7441 (0.1 µM) alone in BGC823 and MGC803 cells. (D) Quantification of protein expression levels. **P<0.01 and ***P<0.0001 vs. NU7441. (E) CX4945 further significantly elevated NU7441 induced cell apoptosis of GC823 cells and MGC803 cells. (F) Quantification of cell apoptosis rates. **P<0.01 and ***P<0.0001. CK2, casein kinase 2; IR, ionizing radiation; γH2AX, γ H2A histone family member X; PI, propidium iodide; FITC, fluorescein isothiocyanate.