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. 2019 Jun 19;42(2):479–494. doi: 10.3892/or.2019.7205

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Matrine improves the inhibitory effect of dexamethasone on C2C12 myoblasts differentiation. (A) Immunofluorescence staining of MHC (green), MyoD (red) and DAPI (blue) in C2C12 myotubes. C2C12 myoblasts were treated with Dex combined with or without Mat in differential medium for 72 h. Quantification of C2C12. (B) Mean myotubes diameter (3–5 measures per myotube; n>150 per group) and (C) Fusion index (n=6). Data are presented as the mean ± standard deviation. Statistical significance was determined by one-way ANOVA. ^#P<0.05 vs. Dex + 0.1 mM Mat. *P<0.05, **P<0.01, ***P<0.001 vs. Dex. ^&&P<0.01, ^&&&P<0.001 vs. NC. MHC, myosin heavy chain.