. 2019 Jun 1;9(6):1183–1200.

Table 1.

Primer lists for PCR and qRT-PCR

Gene	Primer Sequence (5’-3’)	Annealing temperature	Product Size
sgRNA	GTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACC	63°C	150 bp
sgRNA	ATTTTAACTTGCTAGGCCCTGCAGACATGGGTGATCCTCATGTTGGCCTAGCTCTAAAAC	63°C	150 bp
GAPDH	GCCAAAAGGGTCATCATCTC	58°C	287 bp
GAPDH	GTAGAGGCAGGGATGATGTTC	58°C	287 bp
CDKN1A	TGTCCGTCAGAACCCATGC	58°C	139 bp
CDKN1A	AAAGTCGAAGTTCCATCGCTC	58°C	139 bp
TSPAN4	GTACCTGACTCCTGCTGCTT	58°C	116 bp
TSPAN4	AGCAGGTTCTCCTGAAGC	58°C	116 bp
ELAVL2	TCTTCTGCCTCAATTCGC	52°C	139 bp
ELAVL2	CAATGACCCAGAAGGAGTTG	52°C	139 bp
JUNB	CAAGGTGAAGACGCTCAAGG	58°C	95 bp
JUNB	TCATGACCTTCTGTTTGAGCTG	58°C	95 bp
PAAF1	GATGCCCAGCTGAAGATATG	58°C	120 bp
PAAF1	AGCAGACACCACATTCCTCC	58°C	120 bp