Figure 1.

TMZ-treated GBM cells strongly impaired expression of pro-inflammatory cytokines. A, B. PBMC were preactivated with Anti-CD3/CD28 beads for 12 h, and co-cultured with U87 cells which were pretreated with or without 200 μM TMZ. Then the culture medium was collected and subjected to IFN-γ and IL-2 quantification by ELISA after 24 h co-incubation. C, D. The activated PBMC were co-cultured with U251 cells which were challenged with or without 50 μM TMZ. Then IFN-γ and IL-2 protein level in co-culture medium were measured by ELISA after 24 h co-incubation. *P < 0.05, on the basis of Student’s t test.