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The expression levels of immune checkpoints in GBM cells after TMZ challenge. A-D. U87 cells treated with 200 μM TMZ for different times, were collected for measuring mRNA expression levels of CD274, HVEM, Galectin-9 and CD276 by realtime PCR. E-H. After treated with 50 μM TMZ for 0-72 h, U251 cells were collected for evaluating mRNA expression levels of CD274, HVEM, Galectin-9 and CD276 by realtime PCR. *P < 0.05, on the basis of Student’s t test.
