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. 2019 Jun 28;13:287. doi: 10.3389/fncel.2019.00287

FIGURE 1.

FIGURE 1

Representative injection sites at the DRt depicted in coronal sections. (A) Diagram depicting the location of the dorsal reticular nucleus (DRt), at 5.60 mm caudal to the interaural line [adapted from Paxinos and Watson (1998)]. (B) Representative photomicrograph of a thionin-stained section illustrating an injection site in the DRt identified by the needle tract surrounded by the dye staining. (C) Photomicrograph of a representative injection site of the control vector LV-EGFP injected at 0.6 μl into the DRt. The injection site includes a central area located within the DRt surrounded by EGFP-labeled neurons (better depicted at higher magnification in the insert). Scale bar in C: 200 μm (B is at the same magnification), scale bar of the enlargement: 50 μm. Abbreviations: Cu, cuneate nucleus; Gr, nucleus gracilis; IR, intermediate reticular nucleus; NTS, nucleus of the solitary tract; Sp5C, spinal trigeminal nucleus, pars caudalis; VR, ventral reticular nucleus.