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. 2019 Jul 3;2:252. doi: 10.1038/s42003-019-0492-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Trans-species rescue of alcohol sedation in Cp1 RNAi flies. a, c Ethanol sedation in flies with repo-Gal4 alone, HMS00725 alone, UAS-GA25021 transgenes alone, and repo-Gal4 with UAS-GA25021. Genotype did not impact ST50 values (panel a: one-way ANOVA, p = 0.4855, n = 8; panel c: one-way ANOVA, p = 0.1683, n = 8). b, d Ethanol sedation in flies with concurrent expression of Cp1 RNAi and GA25021. ST50 values were decreased in flies constitutively expressing the HMS00725 Cp1 RNAi transgene in all glia via repo-Gal4 (blue squares) compared to control flies containing repo-Gal4 alone (black circles). ST50 values in flies that expressed a UAS-GA25021 transgene and HMS00725 Cp1 RNAi in all glia via repo-Gal4 (gray triangles: UAS-GA25021 #1, panel b; UAS-GA25021 #3, panel d) were significantly elevated compared to flies expressing HMS00725 alone (blue squares: UAS-GA25021 #1, panel b; UAS-GA25021 #3, panel d), but were not different than control flies containing repo-Gal4 alone (black circles) (panel b: one-way ANOVA, p < 0.0001, n = 8,; panel d: one-way ANOVA, p = 0.0019; *Bonferroni’s multiple comparison vs. repo-Gal4;HMS00725 flies, p < 0.05). e–j Whole-mount brain images immunolabeled for Cp1. Whole-brain fluorescence was reduced in flies constitutively expressing the HMS00725 Cp1 RNAi transgene in all glia via repo-Gal4 (f) compared to brains that contained repo-Gal4 alone (e). Compared to brains that contained repo-Gal4 alone (e), whole-brain fluorescence was increased when a UAS-GA25021 transgene was expressed in all glia via repo-Gal4 (UAS-GA25021 #1, panel g; UAS-GA25021 #3, panel i). Compared to brains that expressed the HMS00725 Cp1 RNAi transgene in all glia via repo-Gal4 (f), whole-brain fluorescence was increased when a UAS-GA25021 transgene was expressed with the HMS00725 Cp1 RNAi transgene in all glia via repo-Gal4 (UAS-GA25021 #1, panel h; UAS-GA25021 #3, panel j). Representative images from middle sections of adult brains, ×10 (Anti-Cp1 1:250; Alexa 568 1:1000)