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. 2019 Jul 3;9:9602. doi: 10.1038/s41598-019-46092-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative results of the elemental analysis of cell wall, tyloses and starch grains. Carbon (C) and oxygen (O) distribution in cell wall, tyloses and starch grains observed in stem cross-sections of Leccino and Cellina di Nardò. The atomic percentages of C and O in the observed grains showed statistically significant differences in comparison with the cell wall composition of the xylem vessel according to Student t-test (*P < 0.05; **P < 0.01). The observed masses hypothesized to be tyloses exhibited no differences when compared with the cell wall composition.