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. 2019 Jul 3;9:9636. doi: 10.1038/s41598-019-45642-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of transfection’s efficiency in PND3 ovaries. (a) Representative images of PND3 ovary, after 2 days of transfection with Alexa Fluor® 555-labeled, dsRNA/ Lipofectamine RNAiMax. PND3 ovarian sections (10 µm) showed nuclear labelling with Hoechst (blue) and Alexa Fluor® 555-labeled, dsRNA (red). The merged images indicate that the dsRNA was been successfully transferred into the ovaries. (b) Magnified images indicate that after transfection the labelled-dsRNA is located mainly in stroma and granulosa cells (bold arrow) and in the oocyte of some follicles (dashed arrow). (c) Expression levels of let-7a after transfection with let-7a mimic using liposome-delivery system. The fold change of let-7a is significantly higher in PND3 ovaries compared to control conditions (N = 3), *p-value < 0.05. The error bars represent the standard error. (d) Expression levels of genes involved in apoptosis in four different groups: control, chemotherapy alone (1 h/4-HC/20 µM), let-7a mimic alone (let-7a mimic), chemotherapy + let-7a mimic ((1 h/4-HC/20 µM) + let-7a mimic). STAT3 and BAX are significantly downregulated in the control + let-7a group (N = 5). BAX is significantly downregulated in the chemo + let7-a group (N = 8).