Table 2.
The identification of host ERAD (endoplasmic reticulum-associated protein degradation) and symbiont SELMA (symbiont-derived ERAD-like machinery) components in N. oceanica (NO), N. gaditana (NG), P. tricornutum (PT), C. merolae (CM), and G. sulphuraria (GS)
| Species | NOa | NGa | PTa | CMa | GSa | ||||
|---|---|---|---|---|---|---|---|---|---|
| H | S? | H | S? | H | S | ||||
| ER translocation | Sec61 | X | − | X | − | X | − | X | X |
| Derlin protein | Dfm1, hDer1-1 (sDer1-1) | X | Xsp | X | X | X | X | X | X |
| Der1, hDer1-2 (sDer1-2) | X | − | X | − | X | X | X | X | |
| Ubiquitinylation | Hrd1/Der3 (ptE3p) | X | − | X | − | X | X | X | X |
| Hrd3 | − | − | − | − | X | − | X | X | |
| Uba1 (sUba1) | X | X | X | X | X | X | X | X | |
| Doa10 | X | − | X | − | X | − | X | X | |
| Ubc6 (sUbc6) | Xsf | − | X | − | X | X | X | X | |
| Polyubiquitin (sUbi) | X | − | X | − | X | X | − | − | |
| Cdc48 complex | Cdc48 (sCdc48-1) | X | − | X | − | X | X | X | X |
| (sCdc48-2) | − | − | − | − | − | X | − | − | |
| Ufd1 (sUfd1) | X | − | X | − | X | X | X | X | |
| Npl4 (sNpl4) | X | − | X | − | X | X | X | X | |
| (sUBX) | − | − | − | − | − | X | X | X | |
| (sPUB) | − | − | − | − | − | X | − | − | |
| Processing | Png1 (sPng1) | X | X | X | X | X | X | X | X |
| Dsk2 (sUbq) | X | X | − | X | X | X | X | X | |
| Rad23 | X | − | X | − | X | − | X | X | |
| Ufd2 | X | − | X | − | X | − | X | X | |
| (ptDUP) | − | − | − | − | − | X | − | − | |
| Unknown function | (PPP1) | X | − | X | − | − | X | X | X |
| Chaperones | Hsp70 (sHsp70) | X | Xsp | X | X | X | X | X | X |
| Hsp40/Ydj1/MAS5 | X | − | X | − | X | − | X | X | |
| (sDPC) | − | − | − | − | − | X | − | − |
The genes of N. oceanica are searched against the genome obtained in this study, while those of N. gaditana are searched against the genome published early19 (e-value < e−5 and annotated as the genes searched). In parentheses, symbiont specific if with s or putatively symbiont specific if without s. X, found; –, not found; ?, not sure as the signal peptide at the N-terminal was not found except two with superscript sp of N. oceanica. sf, annotated as different names but the function is the same
aFor these two species, host-specific (H) and symbiont-specific (S) are judged according to the similarity between protein query and its hit. Data cited from Stork et al.42