Fig. 5.

Comparison between nanopore sequencing runs with different DNA fragment sizes. We compared five nanopore sequencing runs of equivalent sequencing quality to understand how input DNA size affects sequencing throughput. a Number of 1D² sequencing reads generated in different sequencing runs. We found a modest decrease in the number of 1D² reads as the input DNA size increased. b 1D² sequencing yield (# of bases) generated in different sequencing runs. We found that large assembly sizes resulted in higher sequencing yield and higher coverage