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. 2019 Jul 3;6:104. doi: 10.1038/s41597-019-0049-y

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© The Author(s) 2019

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Fig. 2 — Demonstration of guided landmark-based measurement of egg shape traits. (a) An example micrograph of an egg, in this case from the cricket Gryllus bimaculatus. (b) The user places points L1 and L2 at the poles of the egg. We define egg ‘poles’ as the points on opposite sides of the egg where the curvature of the egg margin is steepest. The tool draws a line segment connecting L1 and L2 (length) and then draws its perpendicular bisector (dashed blue line). (c) The user uses the blue line as a guide to place points M1 and M2 where the line meets the egg margin. The tool draws a line segment connecting M1 and M2 (q₂). (d) The tool draws a curved segment connecting the midpoint of q₁ with L1 and L2, and then draws two perpendicular bisectors of the curved segment (dashed blue lines). (e) The user uses the blue lines as a guide to place points 1Q1, 1Q2, 3Q1, and 3Q2 where the lines meet the egg margin. The tool draws two lines connecting these points (q₁ and q₃). The user places points S1 and S2 at the ends of the scale bar. (f) Collected measurements from this image are as follows: Length is the distance from L1 to L2. Asymmetry is the ratio of the larger distance among q₁ and q₃ to the smaller. Angle of curvature is calculated as the angle formed by points L1, L2 and the midpoint of q₂. Width is the longest distance between q₁, q₂, and q₃. Aspect ratio is the ratio of length to width. See Tables 6 and 7 for additional details.