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. 2019 Jul 3;9:9622. doi: 10.1038/s41598-019-46075-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophages increased F-actin levels and αSMA incorporation in actin fibers. (a) Representative images of αSMA and F-actin staining in HO2 fibroblasts following 4 days culture in gels in the presence of 10% serum (10%FBS), and in serum-free medium without (SF) and with macrophages (SF + mΦ). Scale bar, 25 µm. Merge images show αSMA (green), F-actin (red), and DAPI (blue). (b) αSMA distribution patterns in HO2 fibroblasts (>100 cells, n = 3). (c,d) αSMA (c) and F-actin (d) levels in HO2 cells in gels in the presence of 10% serum, and in serum-free medium with/without macrophages; shown are fluorescence intensity measurements from maximum intensity projections normalized to the value of serum-free condition (n = 4, *p < 0.05, Mann-Whitney test).