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. 2019 Jul 3;9:9603. doi: 10.1038/s41598-019-45794-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of GUS and LUC driven by truncated Ghd8 promoters. (a) Schematic diagram of the reporter gene GUS driven by the truncated promoters of Ghd8 that were used to generate the transgenic GUS lines. Pro13-1161 represents the truncated promoter of Ghd8 from Nipponbare (Pro13-1469). Pro3-1161 and Pro3-1279G indicate the truncated promoters from ZS97 (Pro3-1469). Pro3-1279T indicates a G replacement of T at the −1279 site of Pro3-1279G, where a predicted pyrimidine box is shown. The GUS expressions in 35-day-old seedling leaves of the corresponding transgenic lines, as indicted in the right panel. (b) Schematic diagram of constructs with the truncated promoters Pro3-1161, Pro3-1279G and Pro3-1279T for luciferase assays in rice protoplasts. (c) Relative firefly luciferase activity to Renilla luciferase activity of Pro3-1161, Pro3-1279G and Pro3-1279T. (d) GUS expression of Pro3-1279G and Pro3-1279T transgenic plants under the treatments of 0 mM (as CK) and 20 mM exogenous GA3. ** and * indicate significant differences among the indicated lines by ANOVA at p < 0.01 and p < 0.05 levels, respectively. Error bars indicate the mean ± SD with three replicates.