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. 2019 Jul 3;9:9641. doi: 10.1038/s41598-019-45997-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GnRH receptor (R) mRNA is detectable in the placental disk at embryonic (e) days 10.5 and 12.5 and the GRIC Cre mediates recombination in maternal decidua and placenta. (A) RT-PCR was performed on placental disks from embryonic days 10.5 and 12.5. Levels of GnRHR were comparable with those seen in adult female pituitaries collected at proestrus/estrus. N = 3 placentas/gestational age. In (B) Adult ROSA26^GRIC+ mice expressing a β-galactosidase (β-gal)-GFP fusion protein show β-gal activity in the female pituitary in situ. β-gal staining indicates the presence of GRIC Cre activity in placenta from e18.5 ROSA26^GRIC+ embryos but not placentas from e18.5 ROSA26^GRIC− embryos. (C) Liquid β-gal activity assay confirmed GRIC Cre-mediated recombination in ROSA26^GRIC+ pituitaries and placentas. ROSA26^GRIC− placental disk and ROSA26^GRIC+ hypothalamus, ovary, pancreas, liver and muscle were used as controls. (D) qRT-PCR showed enrichment of GnRHR mRNA expression in placental decidua compared to whole placental disk. Differing letters (a and b) over individual bars identify differences (p < 0.05). N = >4 placentas/genotype.