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. 2019 Jul 3;9:9585. doi: 10.1038/s41598-019-45632-y

Figure 2.

Figure 2

Confocal images of endocytosis pathways. In MCF-7 cells, the NPs enter the cells through clathrin-dependent and caveolin-dependent endocytosis (A–C) after treatment with 1 mg/mL coumarin-6-labeled NPs for 3 h. Clathrin, caveolin and EEA1 were detected by primary antibodies against clathrin, caveolin and EEA1. (D–F) DsRed-Rab5, 7 and 9 cells were incubated with 1 mg/mL coumarin-6-labeled NPs for 3 h. (F) 1 mg/mL coumarin-6-labeled NPs transfected MCF-7 cells for 3 h and detected by Lyso-Tracker Red probes for 30 min. The above images are the enlarged ones in the white collar on the underside images. Scale bars: 10 μm.