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. 2019 Jul 3;9:9585. doi: 10.1038/s41598-019-45632-y

Figure 4.

Figure 4

Confocal microscopy studies autophagy induced by NPs. (A) EGFP-LC3 cells were cultured with 1 mg/mL Coumarin-6-labeled NPs for 3 h. Western blotting of the cells cultured in (A). (B) DsRed-LC3 cells were cultured with 1 mg/mL Coumarin-6-labeled NPs for 3 h. (C) MCF-7 cells were cultured with 1 mg/mL NPs for 3 h, and P62 was detected with a primary antibody against P62. (D) The autophagosomes fused with lysosomes (arrows). (E) EGFP-LC3 cells were cultured with 1 mg/mL NPs for 3 h and Lyso-Tracker Red probes for 30 min. The above images are the enlarged ones in the white collar on the underside images. Scale bars: 10 μm.