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Validation of the protein evidence for NHERF1. (A) NHERF1 was analyzed by Western blotting using placenta tissue and trophoblast isolated from individual donors. (B) Relative band intensity was estimated based on the average levels obtained from three hPN samples. (C) Antibody-captured signal band was identified as bona fide NHERF1 by LC-MS/MS. The unique peptide of NHERF1 is illustrated.
