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. 2019 Jun 22;10(7):1605–1611. doi: 10.1111/1759-7714.13122

Figure 1.

Figure 1

Representative western blot analyses for IgG and IgM radiation‐induced tumor immunity (RITI) response. Panels (a) and (b) represent western blot analysis of patient serum probed against mixed cell lysates from lung squamous cell carcinoma cell lines H520, H2882, and H2170 for (a) IgG and (b) IgM for a 75‐year‐old woman with stage IB lung squamous cell carcinoma treated with stereotactic body radiation therapy (RT) with photons to a dose of 60 Gy in 10 fractions. (a) No new IgG bands were detected. (b) IgM response weighing 50–75 kDa was detected day 14 following RT start and persisted to at least 3 months post‐RT (depicted by arrows). Panels (c) and (d) represent a western blot analysis of patient serum probed against mixed cell lysates from lung squamous cell carcinoma cell lines H520, H2882, and H2170 for (c) IgG and (d) IgM for a 71‐year‐old man with stage IIIA squamous cell carcinoma of the lung treated with proton RT to a dose of 74 Gy (RBE) in 37 fractions with concurrent chemotherapy. (c) No new IgG bands were detected. (d) New IgM band weighing 37–50 kDa was detected 3 months (Mos) post‐RT (depicted by arrows).