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. 2019 Jun 27;10:1472. doi: 10.3389/fmicb.2019.01472

FIGURE 5.

FIGURE 5

A. thaliana Dgat1 expression induces LD formation in Ole18-PAF102 yeast cells (A) Gene construct introduced into the pGAPZA vector for producing A. thaliana Diacylglycerol o-acyltransferase (Dgat1) enzyme (At2g19450) in P. pastoris. (B) Confocal microscopy images of Ole18-GFP cells with or without the Dgat1 construct, as indicated. Bright field (BF) and fluorescence merged images are shown. (C) Total number of LDs per 30 cells of the indicated strains. LDs were counted in three replicates. Asterisk denotes statistically significant differences (Tukey test, p < 0.005) (D) Confocal microscopy images of Nile red stained Ole18-PAF102 cells with or without the Dgat1 construct as indicated. Bright field (BF), and fluorescence and BF merged images are also shown. Images correspond to single focal sections. (E) Total number of LDs per 30 cells of the indicated strains. LDs were counted in three replicates. Asterisk denotes statistically significant differences (Tukey test, p < 0.005) (F) Distribution of range sizes (area in μm2) from 50 LDs from strain cells indicated. (G) Growth of indicated strain cultures. Graph represents the mean OD600 ± SD value of triplicate cultures measured every 2 h. Bars = 2 μm.