Table 2.

Detailed aspects of immunohistochemistry.

Protein	Antigen retrieval	Antibody	Clonality	Dilution, incubation time, and temperature
CD44	Citrate (0.01 M, pH = 6.0), 98°C, 20 min	MCA2726 AbD Serotec	Monoclonal (156-3C11)	1:2000, 2 h, RT
GLUT1	Citrate (0.01 M, pH = 6.0), 98°C, 20 min	ab15309 Abcam	Polyclonal	1:500, 2 h, RT
CAIX	Citrate (0.01 M, pH = 6.0), 98°C, 20 min	ab15086 Abcam	Polyclonal	1:2000, 2 h, RT
HKII	EDTA (1 mM, pH = 8.0), 98°C, 20 min	ab104836 Abcam	Monoclonal (3D3)	1:1000, 2 h, RT
LDHV	EDTA (1 mM, pH = 8.0), 98°C, 20 min	ab101562 Abcam	Monoclonal (EPR1564)	1:6000, 2 h, RT

RT, room temperature.